Journal: Data in Brief

Article Title: Characterization of polymeric nanoparticles for treatment of partial injury to the central nervous system

doi: 10.1016/j.dib.2016.02.019

Figure Lengend Snippet:

Article Snippet: How data was acquired , Fluorescence microscopy (Nikon Eclipse Ti inverted microscope), nanoscale secondary ion mass spectroscopy (NanoSIMS-CAMECA NanoSIMS 50 ion microprobe at the University of Western Australia)..

Techniques: Fluorescence, Microscopy, Inverted Microscopy, Mass Spectrometry, Western Blot, Incubation, Polymer

Journal: Nature Communications

Article Title: Calorie restriction increases insulin sensitivity to promote beta cell homeostasis and longevity in mice

doi: 10.1038/s41467-024-53127-2

Figure Lengend Snippet: A Study design. After 2 months of age, 15 N-labeled mice were kept on AL, 20% CR or HFD for 12 months. B Multi-isotope imaging mass spectroscopy (MIMS) representation technique. C Validation of 15 N incorporation by MIMS imaging and quantification of 15 N/ 14 N ratio in cortical neurons from a 15 N-labeled mouse after chase with 14 N diet for 12 months. Each dot represents a single neuronal nucleus ( n = 21 nucleus from 2 cortical layers of 1 mouse) D MIMS of pancreatic islets from AL, CR, or HFD mice ( n = 3 islets per animal, n = 1 mouse per diet group). E Single cell analysis of 15 N/ 14 N ratios in beta cells at day 0 (no chase) and after 12 months (mo) on AL, HFD, or CR diet. Each dot represents a single beta cell nucleus, pooled from 3 different islets of 1 mouse per diet group. Total of n = 54 AL 0mo, n = 217 AL 12mo, n = 114 HFD 12m0, and n = 194 CR 12mo beta cells were analyzed. The dotted horizontal pink and black lines represent the mean and the lowest 15 N/ 14 N levels found in cortical neurons shown in ( C ). F Estimation of beta cells that are estimated to be LLC or young after 12 months on AL, CR, or HFD. B , D scale bar = 10 microns. The p values for all significantly different comparison are shown. Statistical analysis was conducted using one-way ANOVA with Benjamini, Krieger, and Yekutieli’s post-hoc test ( B ). All data presented as mean ± 95% confidence intervals (C.l.). A was created with BioRender.com. and released under a Creative Commons Attribution-Non-Commercial NoDerivs 4.0 International license.

Article Snippet: Next, the same samples were transferred to a MIMS microscope (50 L SIMS, Cameca, France) and acquisition of isotope maps ( 15 N, 14 N, 12 C, and 32 S) was achieved using a cesium (Cs − ) beam.

Techniques: Labeling, Imaging, Mass Spectrometry, Biomarker Discovery, Single-cell Analysis, Comparison